Introduction:
EasyPrepTM PCR Cleaning kit provide a fast and reliable method to purify DNA fragment from PCR, RFLP, phosphorylation, labeling and any other enzymatic reactions. DNA binds to the membrane in the presence of high concentration of salt. The other components such as nucleotides, enzymes, salts and dyes pass through the membrane and further washed away by Washing Buffer. Pure DNA is eluted with Elution Buffer (Tris buffer, 10 mM, pH8.5) or H2O. DNA fragments from 200 bp to 20 kb can be purified using the DNA mini binding column with up to 90% recovery rate for PCR cleaning.
Kit Contents:
|
Catalog No.
|
DC02-01
|
DC02-02
|
|
DNA mini Columns
|
50
|
250
|
|
Buffer PB*
|
25 ml
|
125 ml
|
|
DNA Wash Buffer (Concentrate)
|
12 ml
|
54 ml
|
|
Column Buffer
|
6 ml
|
30 ml
|
|
Elution Buffer
|
10 ml
|
20 ml
|
|
2ml Collection Tubes
|
50
|
250
|
|
User Manual
|
1
|
1
|
*Buffer PB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer PB may form precipitates under cool ambient condition. Warm up the buffer at 37oC to dissolve before use.
Protocol:
