Sepsis Panel

Bio-Speedy Sepsis qPCR MX-30 Panel

Sepsis causes prolonged hospitalizations and deaths. Appropriate treatment and rapid recovery process are possible with early diagnosis of sepsis agent. The chance of living with sepsis decreases suddenly as the treatment initiation is delayed. If a patient receives the correct antimicrobial treatment within the first hour of diagnosis, the chance of survival is close to 80%; this rate decreases by 7.6% for each delayed hour.

Intended use: Bio-Speedy® Sepsis qPCR MX-30 Panel is a multiplex kit designed to detect and differentiate 5 fungal, 17 bacterial, and 9 antimicrobial resistance gene agents.

Targets: Neisseria meningitidis, Enterobacteriaceae, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Haemophilus influenzae, Klebsiella oxytoca, Escherichia coli, Stenotrophomonas maltophilia, Pseudomonas spp., Streptococcus spp., Streptococcus pneumoniae, Staphylococcus spp., Staphylococcus aureus, Listeria monocytogenes, Enterococcus faecium, Enterococcus faecalis, VanA - Vancomycin resistance, VanB - Vancomycin resistance, mecA+C - Methicillin resistance, OXA-48 - Carbapenem resistance, KPC - Carbapenem resistance, NDM - Carbapenem resistance, VIM - Carbapenem resistance, IMP - Carbapenem resistance, Candida glabrata, Candida tropicalis, Candida krusei, Candida albicans, Candida parapsilosis.

Operation and Duration: Bio-Speedy® Sepsis qPCR MX-30 Panel provides Real-Time PCR results in a less than 60-minute protocol, after nucleic acid extraction from whole blood or positive blood culture samples. In a 96-well instrument, 10 sample results can be obtained simultaneously within the Negative and Positive controls.

Validated and Compatible Devices: Bio-Speedy® Sepsis qPCR MX-30S Panel is validated with robotic extraction systems such as Zybio EXM3000 Nucleic Acid Isolation System (Model No: EXM3000).

The RT-qPCR is carried out in 20 μL reaction volume using the CFX96 Touch™/CFX96™ Dx/CFX Opus 96™/CFX Opus 96™ Dx (Bio-Rad) Real-Time PCR detection systems equipped with the FAMHEXROX, and CY5 detection channels.

Sample Type: Whole Blood, Positive Blood Culture


Sensitivity: 82%
Specificity: 98.3%
LoD: 500-1000 cfu/mL
Sensitivity: 97.1%
Specificity: 99.3%
LoD: 100-500 cfu/mL
Bio-Speedy® Sepsis qPCR MX-30 Panel
  • • VanA - Vancomycin resistance
    • VanB - Vancomycin resistance
    • mecA+C - Methicillin resistance
    • OXA-48 - Carbapenem resistance
    • KPC - Carbapenem resistance
    • NDM - Carbapenem resistance
    • VIM - Carbapenem resistance
    • IMP - Carbapenem resistance

• Candida glabrata
• Candida tropicalis
• Candida krusei
• Candida albicans
• Candida parapsilosis

Gram (-) Bacteria • Neisseria meningitidis
• Enterobacteriaceae
• Pseudomonas aeruginosa
• Klebsiella pneumoniae
• Acinetobacter baumannii
• Haemophilus influenzae
• Klebsiella oxytoca
• Escherichia coli
• Stenotrophomonas maltophilia
• Pseudomonas spp.
Gram (+) Bacteria


 Streptococcus spp.
 Streptococcus pneumoniae
 Staphylococcus spp.
 Staphylococcus aureus
 Listeria monocytogenes
 Enterococcus faecium
• Enterococcus faecalis


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