T&A Cloning Kit (20 preps)

Price: $185.00


Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.

The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.

  • Fast ligation, completed in only 5 minutes
  • More accurate results
  • Accept a wide range of inserts with different sizes
  • Two types of ligation buffers provided for your convenience
  • Allow blue/white screening
  • Contain ampicillin marker for antibiotic selection
  • Include M13 primer sites for convenient sequencing
  • Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb

    Quality Control

  • DNA concentration of T&A™ Cloning Vector is 25 ng/μl
  • The absorbance ratio (A260/A280) is between 1.6~2.0
  • The size of T&A™ Cloning Vector is about 2.7 kb
  • The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 108cfu/μg DNA
  • The colony number ratio of self-ligation control to positive control is less than 15%
  • The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 108cfu/μg DNA
  • The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5%

Store at

 # FYC001-20P (20 preps) 
T&A™ Cloning Vector 40 μl (25 ng/ μl)
Control Insert DNA 10 μl (10 ng/ μl)
yT4 DNA Ligase 20 μl (2U/ μl)
10X Ligation Buffer A 50 μl
10X Ligation Buffer B 50 μl
Forward Primer (M13-F) 50 μl (10 μM)
Reverse Primer (M13-R) 50 μl (10 μM)

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  • Shipping Weight: 20 lbs

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