5X All-In-One 1st Strand cDNA Synthesis Mix is an optimized ready-to-use mastermix, which eliminates multiple component additions. The mastermix provides sensitive and reliable cDNA synthesis over a range of input RNAs. The size of target cDNA is up to 9kb. Composition: PowerScript™ Reverse transcriptase, Ribonuclease inhibitor, dNTPs, Oligo(dT)s, Random primers in optimized reaction buffer.  PowerScript™ Reverse Transcriptase: a specially engineered RTase based on native RTase from MMLV, (1) No RNase H activity to prevent RNA degradation; (2) Contains a fidelity-enhancing subunit to ensure accuracy in reverse transcription.  Size: 200 µl; 3x1ml Nuclease-free H2O Storage: -20ºC Applications Generation of templates for RT-PCR and qPCR cDNA synthesis from ssRNA cDNA library construction Generation of probes for hybridization DNA primer extension

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PowerScript™ Plus Reverse Transcriptase: a novel recombinant RTase (1) Higher efficiency, capable of synthesize cDNA up to 15kb, (2) Thermostable, the reverse transcription could be performed at high temperature (50-55ºC) to eliminate secondary structure of GC rich region, (3) Contains a fidelity-enhancing subunit, which provides outstanding proofreading. PowerScript™ Plus cDNA Synthesis Mastermix is a proprietary 2x mixture of all component required for first-strand cDNA synthesis, except RNA. It provides highly efficient and specific reverse transcription, even from low-abundance transcripts. The unique blend of random primers and oligo(dT) primers ensures optimal representation of all transcripts in the cDNA product. It synthesize cDNA up to 15kb. Composition: (1) PowerScript™ Plus Reverse Transcriptase (200U/µl, 30µl); (2) 2xReaction mix contains Ribonuclease Inhibitor, dNTPs, Oligo(dT)s and Random Primers, 350µl; (3) 1ml Nuclease-free H2O. Applications: Generation of templates for RT-PCR and qPCR cDNA synthesis from ssRNA cDNA library construction Generation of probes for hybridization DNA primer extension

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