| Transfer Buffer | Item | Application | Storage |
| 10x Tris Glycine | Tris 25 mM, Glycine 192 mM, pH 8.3 | SDS-PAGE gels | 4°C or RT |
| 25x Tris Glycine | Tris 12 mM, Glycine 96 mM, pH 8.3 | Novex® Tris Glycine gels | 4°C or RT |
| 20x Transfer Buffer | Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2 | NuPAGE® gels | 4°C or RT |
Gel Transfer Buffers
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10xTris-Glycine Transfer Buffer (1 L)
1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes. Usage Recommendations: To prepare 1X TG transfer buffer: add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well. Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. MSDS
$22.50
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10xTris-Glycine Transfer Buffer (4 L)
1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes. Usage Recommendations: To prepare 1X TG transfer buffer: add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well. Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. MSDS
$64.50
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10xTris-Glycine Transfer Buffer (10 L)
1xTG: Tris 25 mM, Glycine 192 mM, pH 8.3, with 20% methanol. Western blot transfer buffer Application: For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to membranes. Usage Recommendations: To prepare 1X TG transfer buffer: add 100 ml of 10X TG buffer to 700 ml of deionized water, then add 200 ml methanol and mix well. Use fresh 1X TG transfer buffer. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. MSDS
$122.50
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20xTransfer Buffer for Bis-Tris Gels (500 ml)
Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting. Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.
$40.00
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20xTransfer Buffer for Bis-Tris Gels (1 L)
Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting. Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.
$70.00
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20xTransfer Buffer for Bis-Tris Gels (4 L)
Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting. Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.
$230.00
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25x Tris Glycine Transfer Buffer (1L)
Composition 1x: Tris 12 mM, Glycine 96 mM, pH 8.3 Usage For 1000ml 1xTransfer Buffer, add 40ml 25x, 200ml methanol to 760ml H2O; mix well Safety Data Sheet MSDS
$25.00
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25x Tris Glycine Transfer Buffer (4 L)
Composition 1x: Tris 12 mM, Glycine 96 mM, pH 8.3 Usage For 1000ml 1xTransfer Buffer, add 40ml 25x, 200ml methanol to 760ml H2O; mix well Safety Data Sheet MSDS
$70.00
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25x Tris Glycine Transfer Buffer (10 L)
For Novex gels Composition 1x: Tris 12 mM, Glycine 96 mM, pH 8.3 Usage For 1000ml 1xTransfer Buffer, add 40ml 25x, 200ml methanol to 760ml H2O; mix well Safety Data Sheet MSDS
$105.00
