| Name | 1x Composition | Applications |
| 10x PBS | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, pH 7.4 | Most widely used washing buffer for Western blot, ELISA, Immuno-Histochemistry staining et al. |
| 10x PBST | Phosphate buffer 10 mM, KCl 2.7 mM, NaCl 137 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemical staining et. al. |
| 10x TBS | TBS01: Tris 50 mM, NaCl 150 mM, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBS | TBS03, TBS04: Tris 25 mM, NaCl 137 mM, KCl 2.7 mM, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
| 10x TBST | TBST01: Tris 50 mM, NaCl 150 mM, Tween-20 0.05%, pH 7.4 | Western blot, ELISA, Immuno-Histochemistry |
| 20x TBST | TBST03, TBST04: Tris 25mM, NaCl 137mM, KCl 2.7mM, Tween 0.05%, pH 7.6 | Western blot, ELISA, Immuno-Histochemistry |
Washing Buffers
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1x PBS Buffer (10 L)
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. It contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. It is ready to use. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter Safety Data Sheet MSDS
$75.00
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1x PBST Buffer (10 L)
PBST (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. It contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. The solution is ready to use. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition 137 mM NaCl, 2.7 mM KCl, 10mM phosphate buffer and 0.05% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. Safety Data Sheet MSDS
$80.00
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10x PBS Buffer (1 L)
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS
$22.00
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10x PBST Buffer (1 L)
PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS
$25.00
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10x PBS Buffer (4 L)
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS
$70.00
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10x PBST Buffer (4 L)
PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS
$77.00
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10x PBS Buffer (10 L)
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS
$135.00
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10x PBST Buffer (10 L)
PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS
$160.00
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10x TBS Buffer (1 L)
TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS
$25.00
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10x TBS Buffer (4 L)
TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS
$65.00
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10x TBS Buffer (10 L)
TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS
$130.00
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10xTBST Buffer (1 L)
TRIS-buffered saline with Tween-20 (10x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration. Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS
$24.50
