The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
SKU
DRP01-01
DRP01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
RNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
RNA Wash Buffer
12 ml
50 ml
RT
DEPC H2O
10 ml
25 ml
RT
Collection tubes
100
500
RT
Protein Isolation Buffer
40 ml
200 ml
RT
Manual
1
1
-
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