Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences. The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency. Features Fast ligation, completed in only 5 minutes More accurate results Accept a wide range of inserts with different sizes Two types of ligation buffers provided for your convenience Allow blue/white screening Contain ampicillin marker for antibiotic selection Include M13 primer sites for convenient sequencing Applications Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb Quality Control DNA concentration of T&A™ Cloning Vector is 25 ng/μl The absorbance ratio (A260/A280) is between 1.6~2.0 The size of T&A™ Cloning Vector is about 2.7 kb The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 108cfu/μg DNA The colony number ratio of self-ligation control to positive control is less than 15% The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 108cfu/μg DNA The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5% Store At T&A™ Cloning Vector 40 μl (25 ng/ μl) Control Insert DNA 10 μl (10 ng/ μl) yT4 DNA Ligase 20 μl (2U/ μl) 10X Ligation Buffer A 50 μl 10X Ligation Buffer B 50 μl Forward Primer (M13-F) 50 μl (10 μM) Reverse Primer (M13-R) 50 μl (10 μM)   ***** Warning ! This is cold item, product requires to select Overnight shipping! *****

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