The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids. Instead of using proteinase K to digest the biological materials, the kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to the binding columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion. Kit's Contents SKU GD01-01 GD01-02 Storage Preps 50 250 - DNA columns 50 250 RT GD Lysis Buffer 60 ml 260 ml RT DNA Wash Buffer 12 ml 50 ml RT DNA Elution Buffer 10 ml 25 ml RT Collection tubes 50 250 RT Manual 1 1

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The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids. Instead of using proteinase K to digest the biological materials, the kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to the binding columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion. Kit's Contents SKU GD01-01 GD01-02 Storage Preps 50 250 - DNA columns 50 250 RT GD Lysis Buffer 60 ml 260 ml RT DNA Wash Buffer 12 ml 50 ml RT DNA Elution Buffer 10 ml 25 ml RT Collection tubes 50 250 RT Manual 1 1

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The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids. Two protocols are used in this manual, the traditional method is to lyse the red blood cells and remove the debris. It prevents the debris to clog the DNA columns. Thus can be used for larger volume of whole blood samples. The second method is to apply the lysis buffer directly to the whole blood, serum or plasma, which saves time. This method is especially good for small volume blood samples.  After homogenization, the lysates are transferred to the binding columns. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion. Kit's Contents SKU GD02-01 GD02-02 Storage DNA column set 50 250 RT GD Lysis Buffer 60 ml 260 ml RT DNA Wash Buffer (5x concentrate) 12 ml 54 ml RT DNA Elution Buffer 10 ml 25 ml RT Column Buffer 10 ml 30 ml RT Red Blood Cell Lysis Solution* 40 ml 200 ml RT Manual 1 1 *Lysis Buffer contains Chaotropic salts. Wear gloves when handling this solution

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The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids. Two protocols are used in this manual, the traditional method is to lyse the red blood cells and remove the debris. It prevents the debris to clog the DNA columns. Thus can be used for larger volume of whole blood samples. The second method is to apply the lysis buffer directly to the whole blood, serum or plasma, which saves time. This method is especially good for small volume blood samples.  After homogenization, the lysates are transferred to the binding columns. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion. Kit's Contents SKU GD02-01 GD02-02 Storage DNA column set 50 250 RT GD Lysis Buffer 60 ml 260 ml RT DNA Wash Buffer (5x concentrate) 12 ml 54 ml RT DNA Elution Buffer 10 ml 25 ml RT Column Buffer 10 ml 30 ml RT Red Blood Cell Lysis Solution* 40 ml 200 ml RT Manual 1 1 *Lysis Buffer contains Chaotropic salts. Wear gloves when handling this solution

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The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1

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The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1  

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The EasyPrepTM Bacterial gDNA kit provides a rapid and reliable method for isolating high-quality total cellular DNA from a wide variety of bacterial species. Bacterial cells are grown to log-phase and harvested. The bacterial cell wall is digested by lysozyme and Proteinase K. Following lysis, binding conditions are adjusted and the sample is applied to a DNA column. Key to this kit is our proprietary DNA binding system that allows the high efficient binding of DNA to the membrane while proteins and other contaminates are removed under optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. Each DNA column can bind approximately 100 μg genomic DNA and process up to 1 x 109 bacterial cells. The system combines the reversible nucleic acid-binding properties of membrane matrix with the speed and versatility of spin column technology to yield up to approximately 15-30 μg of DNA with an OD260/280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Kit's Contents SKU GD04-01 GD04-02 DNA Mini Columns 50 250 2 mL Collection Tubes 100 500 Buffer TL 15 ml 70 ml Buffer BL 15 ml 70 ml Buffer KB 28 ml 135 ml DNA Wash Buffer 15 ml 3x24ml Glass Beads 1.5 g 7 g Elution Buffer 15 ml 60 ml Lysozyme 50 mg 5x 50 mg Proteinase K 30 mg 5x30mg RNase A (20mg/ml) 270 µl 1.4 ml Manual 1 1  

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The EasyPrepTM Bacterial gDNA kit provides a rapid and reliable method for isolating high-quality total cellular DNA from a wide variety of bacterial species. Bacterial cells are grown to log-phase and harvested. The bacterial cell wall is digested by lysozyme and Proteinase K. Following lysis, binding conditions are adjusted and the sample is applied to a DNA column. Key to this kit is our proprietary DNA binding system that allows the high efficient binding of DNA to the membrane while proteins and other contaminates are removed under optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. Each DNA column can bind approximately 100 μg genomic DNA and process up to 1 x 109 bacterial cells. The system combines the reversible nucleic acid-binding properties of membrane matrix with the speed and versatility of spin column technology to yield up to approximately 15-30 μg of DNA with an OD260/280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Kit's Contents SKU GD04-01 GD04-02 DNA Mini Columns 50 250 2 mL Collection Tubes 100 500 Buffer TL 15 ml 70 ml Buffer BL 15 ml 70 ml Buffer KB 28 ml 135 ml DNA Wash Buffer 15 ml 3x24ml Glass Beads 1.5 g 7 g Elution Buffer 15 ml 60 ml Lysozyme 50 mg 5x 50 mg Proteinase K 30 mg 5x30mg RNase A (20mg/ml) 270 µl 1.4 ml Manual 1 1  

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The EasyPrepTM Yeast gDNA Kit allows rapid and reliable isolation of high-quality total cellular DNA from a wide variety of yeast species. Up to 3 mL of log-phase culture (OD600 of 1.0 in YPD medium) can be processed. The system combines the reversible nucleic acid-binding properties of DNA-binding matrix with the speed and versatility of spin column technology to yield approximately 15-30 μg of DNA with an A260/A280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrepTM Yeast gDNA Kit, except the Proteinase K, RNase A and Lyticase can be stored at 22 -25 °C and are guaranteed for at least 24 months from the dated of purchase. Once reconstituted in water, Proteinase K and Lyticase must be stored at –20°C. Store RNase A at 4°C. Under cool ambient conditions, a precipitate may form in the Buffer YBL/YTL. In case of such an event, heat the bottle at 37°C to dissolve. Store Buffer YTL/ YBL at room temperature. Kit's Contents SKU GD05-01 GD05-02 DNA Mini Columns 50 250 2 mL Collection Tubes 100 500 Buffer YTL 20 mL 100 mL Buffer YBL 20 mL 100 mL Buffer KB 28 mL 135 mL DNA Wash Buffer 30 mL 3 x 36 mL Glass Beads 3 g 15 g Elution Buffer 15 mL 70 mL Buffer SE 30 mL 135 mL PK storage Buffer 5 mL 10 mL Lyticase (Units) 150 750 Proteinase K 30 mg 150 mg RNase A 100 μL 500 μL Manual 1 1  

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The EasyPrepTM Yeast gDNA Kit allows rapid and reliable isolation of high-quality total cellular DNA from a wide variety of yeast species. Up to 3 mL of log-phase culture (OD600 of 1.0 in YPD medium) can be processed. The system combines the reversible nucleic acid-binding properties of DNA-binding matrix with the speed and versatility of spin column technology to yield approximately 15-30 μg of DNA with an A260/A280 ratio of 1.7-1.9. The kit will isolate all cellular DNA, including plasmid DNA. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrepTM Yeast gDNA Kit, except the Proteinase K, RNase A and Lyticase can be stored at 22 -25 °C and are guaranteed for at least 24 months from the dated of purchase. Once reconstituted in water, Proteinase K and Lyticase must be stored at –20°C. Store RNase A at 4°C. Under cool ambient conditions, a precipitate may form in the Buffer YBL/YTL. In case of such an event, heat the bottle at 37°C to dissolve. Store Buffer YTL/ YBL at room temperature. Kit's Contents SKU GD05-01 GD05-02 DNA Mini Columns 50 250 2 mL Collection Tubes 100 500 Buffer YTL 20 mL 100 mL Buffer YBL 20 mL 100 mL Buffer KB 28 mL 135 mL DNA Wash Buffer 30 mL 3 x 36 mL Glass Beads 3 g 15 g Elution Buffer 15 mL 70 mL Buffer SE 30 mL 135 mL PK storage Buffer 5 mL 10 mL Lyticase (Units) 150 750 Proteinase K 30 mg 150 mg RNase A 100 μL 500 μL Manual 1 1  

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EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C. Kit's Contents SKU GD06-01 GD06-02 DNA Columns 50 250 Collection Tubes 100 500 Buffer FG1 40 mL 180 mL Buffer FG2 12mL 50 mL Buffer FG3 15mL 200 mL Buffer CFG1 30 mL 130 mL DNA Wash Buffer 12 mL 54 mL Elution Buffer 15 mL 50 mL RNase A 100 μL 500 μL Manual 1 1  

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EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Storage and Stability All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C. Kit's Contents SKU GD06-01 GD06-02 DNA Columns 50 250 Collection Tubes 100 500 Buffer FG1 40 mL 180 mL Buffer FG2 12mL 50 mL Buffer FG3 15mL 200 mL Buffer CFG1 30 mL 130 mL DNA Wash Buffer 12 mL 54 mL Elution Buffer 15 mL 50 mL RNase A 100 μL 500 μL Manual 1 1  

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