50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.22 µm membrane Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA Composition: 1x: Tris 40 mM, Acetate 40 mM, EDTA 1 mM, pH 8.0 MSDS

Read more less

50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.22 µm membrane Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA Composition: 1x: Tris 40 mM, Acetate 40 mM, EDTA 1 mM, pH 8.0 MSDS

Read more less

50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.22 µm membrane Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA Composition: 1x: Tris 40 mM, Acetate 40 mM, EDTA 1 mM, pH 8.0 MSDS

Read more less

10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

Read more less

10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

Read more less

10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

Read more less

Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

Read more less

Current DNA electrophoresis buffers are mostly restricte to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

Read more less

Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Tris-boric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

Read more less

10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

Read more less

10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

Read more less

10xTris-Glycine SDS Running Buffer is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Composition: 10xTGS: Tris 250 mM, Glycine 1.92 M, 1%SDS, pH 8.3, Western blot running buffer Application: Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units. Usage Recommendations To prepare 1X TGS buffer, add 100 ml of 10X TGS buffer to 900 ml of deionized water and mix well.  MSDS

Read more less